pSEUDO, a genetic integration standard for Lactococcus lactis.

نویسندگان

  • Joao P C Pinto
  • Araz Zeyniyev
  • Harma Karsens
  • Hein Trip
  • Juke S Lolkema
  • Oscar P Kuipers
  • Jan Kok
چکیده

Plasmid pSEUDO and derivatives were used to show that llmg_pseudo_10 in Lactococcus lactis MG1363 and its homologous locus in L. lactis IL1403 are suitable for chromosomal integrations. L. lactis MG1363 and IL1403 nisin-induced controlled expression (NICE) system derivatives (JP9000 and IL9000) and two general stress reporter strains (NZ9000::PhrcA-GFP and NZ9000::PgroES-GFP) enabling in vivo noninvasive monitoring of cellular fitness were constructed.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 77 18  شماره 

صفحات  -

تاریخ انتشار 2011